Journal: Veterinary microbiology

Article Title: Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

doi: 10.1016/j.vetmic.2021.109067

Figure Lengend Snippet: Fig. 1. Establishment of well-differentiated caprine airway epithelial cell (AEC) cultures. Immunofluorescence (A) and scanning electron micrographs (B) of well-differentiated caprine AEC cultures. (A) blue: DAPI, grey: ZO-1, red: β-Tubulin IV. Scale bars: 50 μm. The images present a representative selection of data from three different donors. (B) The micrograph illustrates the cilia at the apical surface of the caprine AEC culture. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Cilia were visualized using Alexa Fluor® 647-conjugated rabbit anti-β-tubulin antibody (1:200, Cell Signaling Technology®).

Techniques: Immunofluorescence, Selection

Journal: Veterinary microbiology

Article Title: Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

doi: 10.1016/j.vetmic.2021.109067

Figure Lengend Snippet: Fig. 2. Susceptibility and cell tropism of influenza D virus (IDV) on caprine epithelial cell cultures. Caprine AEC were apically infected with 10,000 TCID50 of IDV (A) and mock-infected cells served as a control (B). After 96 h, the cell cultures were fixed and immunofluorescence-stained. The pictures were merged to determine cell tropism and reveal IDV having an affinity to ciliated cells. Grey: ZO-1, red: β-Tubulin IV of ciliated cells, yellow: NP-Protein of IDV. The micrographs present a representative set of data generated from three independent donors. Scale bar: 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Cilia were visualized using Alexa Fluor® 647-conjugated rabbit anti-β-tubulin antibody (1:200, Cell Signaling Technology®).

Techniques: Virus, Infection, Control, Immunofluorescence, Staining, Generated